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Research experience

Rockefeller University, New York City, United States                                                   December 2022-present

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Research assistant in the Laboratory of Cellular and Structural Biology (Prof. Dr. Michael Rout/ Prof. Dr. John LaCava) - Internship

 

Investigating the interplay of LINE-1 retrotransposon proteins with mitochondria and their contribution to the production of immune triggering cytoplasmic RNA:DNA hybrids.

 

Core skills obtained: 

  • Mitochondrial isolation by differential fractionation and affinity capture

  • Subcellular fractionation by density gradient ultracentrifugation

  • Immunoprecipitation 

  • Molecular cloning (site directed mutagenesis)

  • Cell culture and transfection of suspension cells

European Institute for the Biology of Ageing, ERIBA, The Netherlands                                  December 2020-October 2022          

 

Research Technician in the Laboratory of Macromolecules and Interactomes (Prof. Dr. John LaCava) 

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  • Gained exposure to a variety of molecular techniques (CRISPR/Cas9, cell culture, western blot, immunoprecipitation, molecular cloning, transfection, fluorescent microscopy, DNA/protein extraction, bacterial transformation) 

  • Designed small-scale experiments (eg. generation of cell lines, vector cloning) 

 

 

Contributed to the following projects:

  • Dissection of the DNAJB1-PRKAca protein interactome by affinity purification and mass spectrometry from patient material with Fibrolamellar Hepatocellular Carcinoma (FLHCC)

  • Comparative Interactomic Analyses of LINE-1 Ribonucleoprotein from Carcinoma Cells

  • The role of LINE-1 retrotransposable elements in neurodegeneration and autoinflammatory diseases

  • Interplay of LINE-1 proteins with mitochondria

 

Collaborations with multiple labs around the world: Rout Lab, Rockefeller University; Yilmaz Lab, MIT; Burns Lab, Dana-Farber Cancer Institute, Dr. Benjamin Greenbaum Lab, Memorial Sloan Kettering Cancer Center; Prof. Dr. Tomas Mustelin Lab, University of Washington

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Collaborations with pharmaceutical industry: Ribon therapeutics, Inc.; ROME therapeutics

University Medical Center Groningen, UMCG, The Netherlands              

November 2021-May 2022     

                                                     

Internship in the Department of Gastroenterology and Hepatology

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Research topic: Investigating the role of GDF7 in liver fibrosis and its effect on liver proliferation 

 

Core skills obtained: 

  • FFPE tissue preparation

  • Immunofluorescence, Immunohistochemistry 

  • Organoid culture 

  • RNA extraction, cDNA synthesis and qPCR 

Biomedical Research Foundation Academy of Athens                        

June 2019-August 2020

                                                         

Internship in the Department of Fundamental Research (Dr. Marios Agelopoulos) 

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  • Performed phylogenetic analysis of sequence data (databases/software used: Ensembl, Ncbi, UniProt, Galaxy, Clustal, iTOL, R) 

  • Identified potential transcription factor binding sites on DNA sequences 

  • Gained knowledge on laboratory procedures (ChIP-seq, cell culture) 

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General Anticancer Oncology Hospital "Agios Savvas"                            

June 2018-July 2018 

 

Summer internship in the Department of Cancer Cytogenetics 

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  • Gained empirical experience on routine procedures of blood analysis for cancer diagnosis and prognosis. 

  • Received training in the following procedures:

    • Genomic DNA extraction from whole blood

    • Cytogenetic analysis (Blood cell culture, Chromosome isolation, Chromosome staining / G-banding, Karyotyping)

    • Fluorescence in situ hybridization (FISH) in lymphocytes from patients with leukemia

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